The amino acid sequence of copper-zinc superoxide dismutase from bakers' yeast.
نویسندگان
چکیده
منابع مشابه
Amino acid sequence of copper-zinc superoxide dismutase from horse liver.
The complete amino acid sequence of copper-zinc superoxide dismutase from horse liver is reported. The molecule consists of 153 amino acids and has a Mr = 16,000. The primary structure was determined by automated and manual sequence analysis on fragments produced by cleavage of the S-carboxymethylated protein with cyanogen bromide and on peptides obtained by digestion with trypsin, thermolysin,...
متن کاملCopper-zinc superoxide dismutase from the marine yeast Debaryomyces hansenii.
We have isolated the cytosolic form of Cu-Zn superoxide dismutase (SOD) from the marine yeast Debaryomyces hansenii. This enzyme has a subunit mass of 18 kDa. The preparation was found to be heterogeneous by IF electrophoresis with two pI ranges: 5.14-4.0 and 1.6-1.8. The enzyme preparation had a remarkably strong stability at pH 6.0-7.0, surviving boiling for 10 min without losing more than 60...
متن کاملcDNA and derived amino acid sequence of the chloroplastic copper/zinc-superoxide dismutase from aspen (Populus tremuloides).
aerobic and many anaerobic are Table 1. Characteristics of the chloroplastic Cu/Zn-SOD from aspen subiect to oxidative stress resulting from the deleterious efv fects of reduced oxygen species. SOD has been identified as a key enzyme in protecting the cells from oxidative damage by catalyzing the dismutation of superoxide radicals (O2-) to hydrogen peroxide and molecular oxygen (Fridovich, 1986...
متن کاملTotal reconstitution of copper-zinc superoxide dismutase.
The total reconstitution of copper-zinc superoxide dismutase by stoichiometric addition of the metals to completely metal-free apoprotein is described. Reconstitution has been monitored by electron paramagnetic resonance (EPR) and absorption spectroscopy, enzymic activity, and acrylamide gel el%ctrophoresis. Under the same conditions, it has been possible to prepare highly active enzymes with c...
متن کاملPrimary structure of copper-zinc superoxide dismutase from Neurospora crassa.
The complete amino acid sequence of copper-zinc superoxide dismutase from Neurospora crassa is reported. The subunit consists of 153 amino acids and has a Mr of 15,850. The primary structure was determined by automated and manual sequence analysis of peptides obtained by digestions of the carboxymethylated and aminoethylated enzyme with trypsin and thermolysin. The protein is devoid of tryptoph...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)43637-x